β-Actin Mouse Monoclonal Antibody

Specifications (926-42212)

  • Size: 100 µL

  • Storage: -20 °C

  • Species Cross-Reactivity: human, mouse, rat, monkey, hamster

  • Target Molecular Weight: 45 kDa

  • Isotype: Mouse IgG2b

  • Specificity/Sensitivity: This antibody detects endogenous levels of β-actin protein.

  • Immunogen: Animals are immunized with a synthetic peptide (KLH-coupled) that corresponds to residues near the amino-terminus of human β-actin.

  • Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide.

    Do not aliquot the antibody.

    Contains sodium azide.

Recommended Dilution

  • Western Blotting: 1:1000

  • In‑Cell Western™ Assay: 1:200

    Fixative: Methanol

Applications

This β-actin primary antibody can be used for detection of β-actin by Western blot, or as a normalization antibody when performing two-color detection. Detection of this primary antibody can be achieved with IRDye® Goat Anti-Mouse or IRDye Donkey Anti-Mouse secondary antibodies.

See (licor.com/support) for detailed protocols.

Example Data

beta actin mouse primary antibody example data
Figure 6. β-Actin was detected in COS-7, HeLa, and NIH 3T3 cell lines. Dilutions of each cell lysate are: 2.5, 1.25, 0.625, 0.313, and 0.156 µg/well. Typical results are shown using Odyssey Nitrocellulose Membranes (P/N: 926-31092) and Intercept® Blocking Buffer (P/N: 927-70000). The β-Actin antibody was used at a 1:1000 dilution and the IRDye 680RD Goat anti-Mouse (P/N: 926-68170) was used at a 1:15,000 dilution (optimal range is 1:5000 – 1:25000). The Chameleon® Duo Pre-stained Protein Ladder (P/N: 928-60000) was used. The Western blot was imaged the AutoScan function on an Odyssey Infrared Imaging System.