Protocols and Webinars for Developing In-Cell Western Assays

In-Cell Western™ Assay Resources

Resources

Handbooks

In-Cell Western™ Assay Development Handbook

Application Guides

In-Cell Western™ Assay: FAQs for Suspension/Non-Adherent Cells Application Guide

Cell Viability Assay with Sapphire700™Stain and the Odyssey® CLx and Sa Imaging Systems Application Guide

Brochures

Solutions for Advanced Scientific Research Brochure

Applications for Advancing Research Brochure

Integrated Imaging Solutions for Reliable Results Brochure

Developing Targeted Therapeutics Brochure

White Papers

Quantitative methods for analysis of protein phosphorylation in drug development white paper

A cell-based immunocytochemical assay for monitoring kinase signaling pathways and drug efficacy white paper

Protocols

Probe Design and in vitro Validation Protocol

Example Experiments

Detecting Feline Panleukopenia Virus in CrFK Cells Using Virus Titration Example Experiment

Measuring Apoptosis in HeLa Cells Following Anisomycin Treatment Example Experiment

Detecting p38 Activation in HeLa Cells Following Anisomycin Treatment Example Experiment

Detecting p53 Activation in COS-7 Cells Following Hydroxyurea Treatment Example Experiment

Characterizing AKT Activation in Response to PDGF-BB Treatment in NIH-3T3 Cells Example Experiment

Characterizing ERK Activation in Response to EGF Treatment in A431 Cells Example Experiment

Characterizing ERK Activation in Response to EGF Treatment in HeLa Cells Example Experiment

Characterizing ERK Activation in Response to FGF Treatment in NIH-3T3 Cells Example Experiment

Characterizing U0126 Inhibition of the FGFR Signaling Pathway in NIH-3T3 Cells Example Experiment

Characterizing ERK Activation in Response to Phorbol 12-Myristate 13-Acetate (PMA) Example Experiment

Webinars

Developing Targeted Therapeutics: What You Need to Know

See some published examples of In-Cell Western Assays

View publications