IRDye 680LT Goat anti-Mouse IgM (mu chain) Secondary Antibody

Immunogen

Mouse IgM paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG₁, IgG_2a, IgG₃, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging

Not Recommended for:

Small Animal Imaging
In-Cell Western Assay
On-Cell Western Assay

Formulation

IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:10,000 - 1:50,000
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 - 0.02%) and Tween^® 20 (final concentration of 0.1 - 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

P/N 925-68080: RRID AB_2814917
P/N 926-68080: RRID AB_2814920

Example Data

IRDye® 680LT Goat anti-Mouse IgM (u chain specific) Secondary Antibody data — **Western blot detection of guinea pig IgG with IRDye 680LT Donkey anti-Guinea Pig.** Serial dilutions of guinea pig IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey^® Nitrocellulose membrane (P/N 926-31092). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 680LT Donkey anti-Guinea Pig (P/N 926-68030).

IRDye® 680LT Goat anti-Mouse IgM (µ chain specific) Secondary Antibody