IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody | LICORbio

IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody

IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody | LICORbio

Immunogen

Rabbit IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western Assay applications.

Applications

Highly recommended for:

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:5,000 - 1:25,000
In-Cell Western Assay detection 1:800 1:200 - 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

  • P/N 925-32211: RRID AB_2651127
  • P/N 926-32211: RRID AB_621843

Selected P/N: 926-32211,925-32211

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*Includes all LICORBio secondary antibodies
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