IRDye 800CW Donkey anti-Chicken Secondary Antibody

Highly cross-adsorbed donkey anti-chicken IgG (H+L) antibody conjugated to IRDye 800CW.

Immunogen

Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

Western Blot
In-Cell Western™ Assay
On-Cell Western Assay
Protein Array
Immunohistochemistry
Small Animal Imaging
Microscopy
2D Gel Detection
Tissue Section Imaging
Virus Titration Assay

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:5,000 - 1:25,000
In-Cell Western Assay detection	1:800	1:200 - 1:1,200
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

P/N 925-32218: RRID AB_2814922
P/N 926-32218: RRID AB_1850023

Example Data

IRDye® 800CW Donkey anti-Chicken Secondary Antibody data — **Detection of actin with IRDye 800CW Donkey anti-chicken.** Odyssey^® One-Color Molecular Weight Marker (P/N 928-40000) and C32 lysate, 3 µg – 0.01 µg (Santa Cruz P/N sc-2205) were resolved on a 10% Bis Tris gel and transferred to Odyssey Nitrocellulose membrane (P/N 926-31092). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with chicken anti-actin (GenWay P/N 15-288-23014) and IRDye 800CW Donkey anti-Chicken (P/N 926-32218).

IRDye® 800CW Donkey anti-Chicken Secondary Antibody