VRDye™ 549 Goat anti-Mouse IgG Secondary Antibody

Highly cross-adsorbed goat (polyclonal) anti-mouse IgG (H+L) antibody conjugated to VRDye 549.

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG₁, IgG_2a, IgG_2b, and IgG₃, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy and flow cytometry applications.

Applications

• Western blotting
• Microscopy
• Immunohistochemistry
• Flow Cytometry

Formulation

VRDye 549 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.